A simple, fast, accurate and precise method has been developed for determination of eperisone hydrochloride from pharmaceutical formulation by Reversed-phase high performance liquid chromatography. The separation was carried out on C18 column using mobile phase consisting of a mixture of acetonitrile and water in the ratio 90:10 v/v (pH adjusted to 3.5 with orthophosphoric acid). The flow rate was maintained at 0.9 ml/min. The UV detection was carried out at wavelength 276 nm. The retention time for eperisone hydrochloride was found to be 3.79 min. Linear response obtained for eperisone hydrochloride in the concentration range 3-15 µg/ml (r2 =0.996). The relative standard deviation was found less than 2% for six replicates. The method was validated according to the ICH guidelines with respect to linearity, precision, accuracy, limit of detection, limit of quantification and robustness. Thus, proposed method can be successfully applicable to the pharmaceutical preparation containing the above mentioned drugs without any interference of excipients.
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